| Filename | Description |
|---|---|
| README.md | This file |
| SPRI-Bead.pdf | Demonstrates composition and use of Magna Beads |
| i5-i7_Index_Primer_Maps_*.xlsx | Plate maps for index primer pairings (used in round 2 of 2-PCR Illumina protocol) |
| Dual_Index_Plate_Map_Template_Autofill.xlsx | Plate map ... enter sample IDs for Illumina barcodes |
| media_recipes_gz.txt | Selecet media recipes used for fungal endophyte isolation and culture |
| Illumina_Protocols.pdf | Current lab protocol for 2-round PCR for target amplification and Illumina library prep |
| 2-Round_PCR_Calculator.xlsx | Master mix calculator for both PCR rounds in Illumina_Protocols.html; Just enter the number of samples |
| GelRed_Protocol.pdf | Protocol for staining and visualizing PCR products on agarose gels using GelRed dye |