Zahn lab protocols

Important File List:

Filename	Description
README.md	This file
SPRI-Bead.pdf	Demonstrates composition and use of Magna Beads
i5-i7_Index_Primer_Maps_*.xlsx	Plate maps for index primer pairings (used in round 2 of 2-PCR Illumina protocol)
Dual_Index_Plate_Map_Template_Autofill.xlsx	Plate map ... enter sample IDs for Illumina barcodes
media_recipes_gz.txt	Selecet media recipes used for fungal endophyte isolation and culture
Illumina_Protocols.pdf	Current lab protocol for 2-round PCR for target amplification and Illumina library prep
2-Round_PCR_Calculator.xlsx	Master mix calculator for both PCR rounds in Illumina_Protocols.html; Just enter the number of samples
GelRed_Protocol.pdf	Protocol for staining and visualizing PCR products on agarose gels using GelRed dye